Spectra Analysis, Inc. Logo 257 Simarano Drive
Marlborough, MA 01752
Phone: 1.508.281.6232
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Hyphenating Infrared Spectroscopy to Gas & Liquid Chromatography

DiscovIR-LC™

 

Spectra Analysis IR Infrared IR-LC2 Detection System

 

Enhanced Resolution
Infrared Detection System for HPLC and GPC

System Overview

 

Operating Principle Direct deposition of column eluent on moving ZnSe sample disc
IR Spectrometer Built-in FTIR
IR Detector 0.1 mm MCT
Wavenumber Range 4000–650 cm-1
Data Collection Real-time, with post-run analysis available
Spectrum Type Transmittance through disc and solid-phase sample
Window Capacity 12-24 hours of chromatography
Window Temperature -100°C to +50°C

 

Data Station

 

Platform Windows™ XP
Spectroscopy Package Grams32
Standard Features • Real-time and post-run data collect
• Chromatographic / spectral workup
• Band chromatograms for chemical classes
• Ratio chromatograms for profiling trends
Library Search Library Search Software included
Spectra compatible with all solid phase FTIR Libraries

 

DiscovIR-LC Configuration

 

Sample Delivery Multi-Step Desolvation Interface
Flow Rates Accepted 0.25 to 1.5 mL/min
Compatible Solvents Water, ACN, Methanol, THF, Chloroform
Compatible Additives Volatile buffers (ammonium carbonates, acetates, formates), trifluoroacetic acid (TFA), triethylamine (TEA)
Sensitivity Typically 1ug per component
Chromatograph Any Liquid Chromatograph System (supplied by user)

DiscovIR-LC™

  • Micro-Deposition on Cryogenically-Cooled IR-transparent disc
  • Unique, Proprietary LC interface
    removes solvent interference
  • Collects real-time IR spectrum
    automatically
  • On-line analysis
  • No sample handling!

Key Applications

  • Deformulation
  • Co-polymer Compositional Drift
  • Extractables & Leachables
  • Minor Component Identification
  • Chemical Troubleshooting
  • Degradation/Failure Analysis

Micro-Deposition Improves Analysis

DiscovIR’s unique temperature controlled, vacuum deposition method ensures that results are accurate and reproducible. Eluted peaks are first deposited in a film on to a rotating IR transparent disc. Next, an infrared beam passes through each concentrated spot and the detector automatically collects spectral data.

Concentrates Analytes in Minimal Area

Cryogenic temperature control of the Sample Disc minimizes the area of the deposited analyte. This results in a thicker sample layer and therefore Higher Sensitivity of the absorbance spectrum.

Immobilizes Entire Run

Since the entire chromatographic run is applied to the Sample Disc, the resolution of the separation is not lost as in some collection systems. Additionally, the entire run is available to be scanned again to investigate subtle features or improve signal-to-noise. The speed of the rotating Sample Disc can be adjusted to match concentration of the deposited sample.