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Enhanced Resolution
Infrared Detection System for HPLC and GPC
System Overview
| Operating Principle |
Direct deposition of column eluent on moving ZnSe sample disc |
| IR Spectrometer |
Built-in FTIR |
| IR Detector |
0.1 mm MCT |
| Wavenumber Range |
4000–650 cm-1 |
| Data Collection |
Real-time, with post-run analysis available |
| Spectrum Type |
Transmittance through disc and solid-phase sample |
| Window Capacity |
12-24 hours of chromatography |
| Window Temperature |
-100°C to +50°C |
Data Station
| Platform |
Windows™ XP |
| Spectroscopy Package |
Grams32 |
| Standard Features |
• Real-time and post-run data collect
• Chromatographic / spectral workup
• Band chromatograms for chemical classes
• Ratio chromatograms for profiling trends |
| Library Search |
Library Search Software included
Spectra compatible with all solid phase FTIR Libraries |
DiscovIR-LC Configuration
| Sample Delivery |
Multi-Step Desolvation Interface |
| Flow Rates Accepted |
0.25 to 1.5 mL/min |
| Compatible Solvents |
Water, ACN, Methanol, THF, Chloroform |
| Compatible Additives |
Volatile buffers (ammonium carbonates, acetates, formates), trifluoroacetic acid (TFA), triethylamine (TEA) |
| Sensitivity |
Typically 1ug per component |
| Chromatograph |
Any Liquid Chromatograph System (supplied by user) |
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DiscovIR-LC™
- Micro-Deposition on Cryogenically-Cooled IR-transparent disc
- Unique,Propietary LC interface
removes solvent interference
- Collects real-time IR spectrum
automatically
- On-line Analysis -
- No Sample Handling!
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Key Applications
- Deformulation
- Co-polymer Compositional Drift
- Extractables & Leachables
- Minor Component Identification
- Chemical Troubleshooting
- Degradation/Failure Analysis
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Micro-Deposition Improves Analysis
DiscovIR’s unique temperature controlled, vacuum deposition method ensures that results are accurate and reproducible. Eluted peaks are first deposited in a film onto a rotating IR transparent disc. Next, an infrared beam passes through each concentrated spot and the detector automatically collects spectral data. |
Concentrates Analytes in Minimal Area
Cryogenic temperature control of the Sample Disc minimizes the area of the deposited analyte. This results in a thicker sample layer and therefore Higher Sensitivity of the absorbance spectrum. |
Immobilizes Entire Run
Since the entire chromatographic run is applied to the Sample Disc, the resolution of the separation is not lost as in some collection systems. Additionally, the entire run is available to be scanned again to investigate subtle features or improve signal-to-noise. The speed of the rotating Sample Disc can be adjusted to match concentration of the deposited sample. |
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